YALIN CAMSI KARBON ELEKTROT KULLANARAK FARMASÖTİK FORMULASYON VE İNSAN İDRARINDAN KORTİZOLÜN HIZLI KARE DALGA VOLTAMETRIK ANALİZİ

Ertuğrul KESKİN

YALIN CAMSI KARBON ELEKTROT KULLANARAK FARMASÖTİK FORMULASYON VE İNSAN İDRARINDAN KORTİZOLÜN HIZLI KARE DALGA VOLTAMETRIK ANALİZİ

Ertuğrul KESKİN

Öz

Ticari ilaçlardan ve insan idrarından kortizolün direkt tayini için basit, hızlı, ve duyarlı bir elektrokimyasal yöntem geliştirildi. Kortizolün elektrokimyasal indirgenmesi ve nicel analizleri, dönüşümlü voltametri (DV) ve kare dalga voltametrisi (KDV) kullanılarak yalın camsı karbon elektrot (CKE) ile Britton-Robinson tampon çözeltisinde (BRTÇ) ve pH 2,0’de gerçekleştirildi. Kortizol, tersinmez ve adsorpsiyon kontrollü bir indirgeme piki gösterdi. Adsorptif kare dalga sıyırma voltametrisi (Ad-KDSV) kullanıldığında bileşik BRTÇ pH 2,0 içinde ve -1,14 V'de (Ag/AgCl'ye karşı) iyi tanımlanmış bir voltametrik yanıt verdi. Deneysel sonuçlar pik akımı ile 1-50 µg mL^-1 (2, 8 x 10^-6mol L^-1-1, 4 × 10^-4 mol L^-1) derişim aralığında doğrusal bir ilişki olduğunu ortaya koymuştur. Gözlenebilirlik sınırı (GS) ve tayin alt sınırı (TAS) sırasıyla 0,069 µg mL^-1 (1,9×10^-7 mol L^-1) ve 0,229 µg mL^-1 (6,32×10^-7 mol L^-1) olarak saptandı. Geliştirilen yöntemin kesinliği ve doğruluğu, tablet ve idrar numunelerinden yapılan geri kazanım çalışmaları ile kontrol edildi.

Anahtar Kelimeler

Kortizol, Adsorptif kare dalga sıyırma voltametrisi, Camsı karbon elektrot, Farmasötik formülasyon, İnsan idrarı

Tam Metin:

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